畜牧兽医学报 ›› 2017, Vol. 48 ›› Issue (11): 2189-2197.doi: 10.11843/j.issn.0366-6964.2017.11.021

• 基础兽医 • 上一篇    下一篇

抑制PI3K/AKT信号通路对鹿茸干细胞的影响

刘振, 孙红梅, 李春义*   

  1. 中国农业科学院特产研究所分子生物学重点实验室, 长春 130112
  • 收稿日期:2017-06-06 出版日期:2017-11-23 发布日期:2017-11-23
  • 通讯作者: 李春义,E-mail:lichunyi1959@163.com
  • 作者简介:刘振(1987-),男,山东聊城人,博士生,主要从事鹿茸分子生物学研究,E-mail:liuzhen19871224@126.com
  • 基金资助:

    国家自然科学基金(31500792);吉林省科技平台项目(20150204071NY)

Differential Effects of Inhibition of the PI3K/AKT Pathway on Antler Stem Cells' Parameters

LIU Zhen, SUN Hong-mei, LI Chun-yi*   

  1. State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Wild Economic Animals and Plants, Chinese Academy of Agricultural Sciences, Changchun 130112, China
  • Received:2017-06-06 Online:2017-11-23 Published:2017-11-23

摘要:

旨在探究PI3K/AKT信号通路在鹿茸干细胞中所发挥的作用,以期为揭示哺乳动物器官发生和完全再生机制提供借鉴。本研究通过MTT分析、细胞周期检测、细胞骨架染色等方法研究了抑制PI3K/AKT信号通路后对鹿茸干细胞增殖、细胞黏附、细胞周期、细胞骨架和促血管形成作用的影响。结果发现:1)相对于PP细胞,PI3K/AKT信号通路在调控AP细胞增殖和维持细胞骨架方面具有更为重要的作用;2) AP细胞条件培养液具有明显的促血管形成作用,PP细胞条件培养液不具有明显促进血管形成的作用。试验结果初步表明:相对于鹿茸再生,PI3K/AKT信号通路在鹿茸发生过程中的调控作用更为重要。

Abstract:

Understanding the role of the PI3K/AKT pathway in regulating basic parameters of antler stem cells, include antlerogenic periosteum (AP) cells and pedicle periosteum (PP) cells, and provide valuable insights into the mechanisms underlying mammalian organ generation and regeneration. In the present study, we investigated the effects of the PI3K/AKT pathway on antler stem cell proliferation, adhesion, cell cycle, cytoskeleton and angiogenesis. Our results showed that:1) PI3K/AKT pathway had a greater influence on proliferation of the AP cells and played a more important role in maintaining the normal cell cytoskeleton than those of the PP cells; 2) conditioned medium of the APCs stimulated tube formation,whereas the PPCs did not significantly do so. Our results suggest that PI3K/AKT pathway may play more important role in antler generation than regeneration.

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